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Media Kedokteran Hewan

ISSN 2015-8930

Vol. 23 / No. 3 / Published : 2007-09

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Original Article :

The role of choline esterase on vesicle brain development of chicken embryo of exposure by carbofuran insecticide

Author :

  1. Epy Muhammad Luqman*1
  2. Bambang Poernomo Soenardirahardjo*2
  3. Laba Mahaputra*3
  1. Bagian Anatomi Veteriner,Fakultas Kedokteran Hewan
    Universitas Airlangga Jl. Muyorejo Surabaya 60115 Telp. 031.5992785 Fax. 031.5993015
  2. Bagian Anatomi, Fakultas Kedokteran Hewan Universitas Airlangga Jl. Muyorejo Surabaya 60115 Telp. 031.5992785 Fax. 031.5993015
  3. Bagian Reproduksi Fakultas Kedokteran Hewan Universitas Airlangga Jl. Muyorejo Surabaya 60115 Telp. 031.5992785 Fax. 031.5993015

Abstract :

Carbofuran exposure during embryonic development inhibit ChE and lead to abnormal brain development. This research was aimed to identify the effect of carbofuran exposure to chicken embryo on brain vesicle development during embryonal stage. This research used Randomized Complete design with 3 treatments and repetition with 10 fertile eggs each. Each egg in the control group were injected 0.1 ml NaCl physiologic 0.09%. All of eggs were stored into incubator in 38oC with 60 – 80 % humidity. Observation to brain vesicle were carried out in 72 hr incubation using whole mounts technique. In 72 hr after incubation, the measurement of ChE concentration were carried out by grinding the brain vesicle and the other eggs adding 0.2 ml sterile NaCl physiologic 0.9% solution.The exposure of carbofuran in degraded dose resulted in the increase ChE which was significantly difference and no significant differences in the formation of brain vesicle in those groups. Increasing ChE concentration caused brain vesicle have responsed to carbofuran as toxic agent and subserve growth regulatory and morphogenetic functions in developing embryo. Although carbofuran can promote cholinergic activity evoke neurodevelopmental damage, but brain vesicle resistant to carbofuran as good as adult brain.

Keyword :

Choline Esterase, Chicken Embryo, Carbofuran,


References :

  1. AP Alvares, (1992). Pharmacology and toxicology of carbamates. In: Clinical and Experimental Toxicology of Organophosphat and Carbamates.. Oxford : Ballantyne B. and T. C. Marrs (ed). Butterworth-Heinemann Ltd
  2. B Ballantyne, (1992). Overview of the biological and clinical aspects of organophosphat and carbamates. In: Clinical and experimental toxicology of organophosphat and carbamates. Oxford : Ballantyne B. and T. C. Marrs (ed). Butterworth-Heinemann Ltd
  3. JW Bigbee, (1999). Morphogenic role for acetylcholinesterase in axonal outgrowth during neural development. Environ : Health Perspect. 107 (S1)
  4. S Brimijoin, (1999). Cholinesterases in neural development: new findings and toxicologic implications. Environ : Health Perspect. 107 (S1)
  5. C Brodie, (1991). Growth factors attenuate the cholinotoxic effects of ethanol during early neuroembryogenesis in the chick embryo. - : Int. J. Dev Neurosci. 9 (3)
  6. California Environmental Protection Agency, (2000). Carbofuran. Public Health Goals for Chemicals in Drinking Water. California : California Environmental Protection Agency. Sacramento
  7. A Dajan, (1986). Pengantar Metode Statistik. Jakarta : Jilid II. LP3S
  8. MD Faiman, (1991). Covalent binding of chick embryo proteins by the alkylthiocarbamate molinate. - : Toxicologist. 11(1)
  9. M Farege-Elawar, (1991). Development of esterase activities in the chick embryo and chicken. - : Toxicologist. 11(1)
  10. WHO FAO, (2000). Thiodicarb. Pesticide residues in food. Toxicology evaluations. FAO and WHO working groups. Food and Agriculture Organization and World Health Organization. . - : United Nation Organization
  11. KA Funk, (1994). Avian embryonic brain reaggregate culture system: Characterization for organophosphorus compound toxicity studies. - : Toxicol. Appl. Pharmacol. 124 (1).
  12. S Gilbert, (1988). Developmental Biology. 2nd ed.. - : Sinauer Associates. Massachuset
  13. O Jin, (1996). Teratogenic synergy between a thiocarbamate herbicide and an organophosphorus insecticide. - : Faseb. J. 10(3)
  14. KBS Kumar, (1992). Teratogenic effects of methyl parathion in developing chick embryos. - : Vet and Human Toxicol. 34(5)
  15. PG Layer, (1991). Choline esterase during development of the avian nervous system. - : Cell Mol Neurobiol. 11
  16. JM Lauder, (1999). Morphogenetic roles of acetylcholine. - : Environ. Health Perspect. 107 (S1)
  17. TL Lassiter, (1999). Is the fetal brain protected from gestational exposure to chlorpyrifos?. - : Neurotoxicology. 20(1)
  18. M Lotti, (1992). Central neurotoxicity and behavioural effects of anticholinesterases. In: Clinical and Experimental Toxicology of Organophosphat and Carbamates. Ballantyne B. and T. C. Marrs (ed). Oxford : Butterworth-Heinemann Ltd
  19. FC Lu, (1995). Toksikologi Dasar. Jakarta : Universitas Indonesia
  20. AH Martin, (1993). Magnetic field effects on 5'nucleotidase: acetylcholinesterase and alkaline phosphatase in developing chick embryos. - : Teratology 48(2)
  21. A Meyer, (2003). Developmental neurotoxicity elicited by gestational exposure to chlorpyrifos: When is aenylyl cyclase a target?. - : Environ Health Perspect 111
  22. M Misawa, (1981). Teratogenic effects of cholinergic insecticides in chick embryos.. Diazinon treatment on acetylcholinesterase and choline acetyltransferase activities. - : Toxicol. Appl. Pharmacol. 57(1)
  23. S Siegel, (1988). Nonparametric Statistics for The Behavioral Science. - : McDrwaa-Hill Book Company
  24. TA Slotkin, (1999). Developmental cholinotoxicants: nicotine and chlorpyrifos. - : Environ. Health Perspect. 107 (S1):
  25. RGD Steel, (1993). Prinsip dan Prosedur Statistika. - : PT Gramedia. Pustaka Utama
  26. RW Tyl, (1992). Development and reproductive toxicity of anticholinesterases. In: Clinical and Experimental Toxicology of Organophosphat and Carbamates. Ballantyne B. and T. C. Marrs (ed). Oxford : Butterworth-Heinemann Ltd
  27. ER Weiss, (1998). Why do neurotransmitters act like growth factors?. - : Perspect. Dev. Neurobiol. 5(4)


   


Archive Article

Cover Media Content

Volume : 23 / No. : 3 / Pub. : 2007-03
  1. Cecal goblet cell response on intracellular development of eimeria tenella in susceptible and infected chickens
  2. Protein characterization of snail mucin (achasin) local isolate as an antibacterial factor
  3. Trypsin and pronase treatment on the development of mouse embryos contaminated by k99 escherichia coli
  4. The optimation of tyrosine kinase activity from sperm isolation of friesian holstein cow
  5. Insulin-like growth factor i (igf-i) complex seminal plasma of goatratio glicoprotein : protein : carbohydrate
  6. The role of choline esterase on vesicle brain development of chicken embryo of exposure by carbofuran insecticide
  7. Marker-sets for paternity and identity test in pigs by meansmicrosatellite analysis
  8. Bcg vaccination increased roi secretion in dogs macrophage infected with mycobacterium tuberculosis
  9. Antifertility effect of aqoueus extract betel nut of areca catechu: as an apoptosis agent on testis connective cells of rattus norvegicus
  10. The morphology of mandibular and lingual glands of chicken (gallus sp) and quail (coturnix coturnix): with special refference to the distributionand carbohydrate content
  11. Detection of rabies virus in saliva and brain using antibody from g protein as material diagnostic by indirect double antibody sandwich elisa technique
  12. Study to rice brand in drying ensilage product from edible snail for decrease higroscopies as feed stuff
  13. Potency rice straw was deammoniated and fermented by celulolytic bacteria on the dry matter consumption, body weight gain and feed conversion of sheep
  14. Cecal goblet cell response on intracellular development of eimeria tenella in susceptible and infected chickens
  15. Protein Characterization Of Snail Mucin (achasin) Local Isolate As An Antibacterial Factor
  16. The role of choline esterase on vesicle brain development of chicken embryo of exposure by carbofuran insecticide
  17. The optimation of tyrosine kinase activity from sperm isolation of friesian holstein cow
  18. Insulin-like growth factor i (igf-i) complex seminal plasma of goat ratio glicoprotein : protein : carbohydrate
  19. Marker-sets for paternity and identity test in pigs by means microsatellite analysis
  20. Bcg vaccination increased roi secretion in dogs macrophage infected with mycobacterium tuberculosis
  21. Antifertility effect of aqoueus extract betel nut of areca catechu: as an apoptosis agent on testis connective cells of rattus norvegicus
  22. Trypsin and pronase treatment on the development of mouse embryos contaminated by k99 escherichia coli
  23. The morphology of mandibular and lingual glands of chicken (gallus sp) and quail (coturnix coturnix): with special refference to the distribution and carbohydrate content
  24. Detection of rabies virus in saliva and brain using antibody from g protein as material diagnostic by indirect double antibody sandwich elisa technique
  25. Study to rice brand in drying ensilage product from edible snail for decrease higroscopies as feed stuff
  26. Potency rice straw was deammoniated and fermented by celulolytic bacteria on the dry matter consumption, body weight gain and feed conversion of sheep
  27. Cecal goblet cell response on intracellular development of eimeria tenella in susceptible and infected chickens