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Media Kedokteran Hewan

ISSN 2015-8930

Vol. 22 / No. 2 / Published : 2006-05

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Original Article :

Neutralization of avian reovirus 1091 in chick kidney and vero cell cultures by monoclonal antibodies against b/bc and c proteins

Author :

  1. Nyoman Mantik Astawa*1
  1. Depatement of Virology, Faculty of Veterinary Medicine, Udayana University, Jl. P.B. Sudirman, Denpasar, Bali, Indonesia

Abstract :

A study on the neutralization of avian reovirus 1091 by monoclonal antibodies (MAbs) against B/BC and C proteins was carried out in primary chick kidney (CK) and secondary Vero (African green monkey kidney) cell cultures. MAbs against individual proteins of avian reovirus 1091 were produced by fusion of spleenocytes derived from mice immune to avian reovirus 1091, with immortal mouse myeloma cells. The neutralization of the virus infectivity by MAbs in primary chick kidney (CK) and Vero (African green monkey kidney) cell cultures was determined by plaque reduction neutralization test (PRNT). Four MAbs against B/BC protein and 5 MAbs C protein of avian reovirus 1091 were produced in this study. Four MAbs against against B/BC were designated as 4D1, 4E2, 5F5 and 5A6. Five MAbs against C portein were designated as 1A2, 4D3, 5A12, 5B11 and 5C6. PRNT showed that one MAb (5A6) against B/BC neutralized the virus infectivity in CK and Vero cell cultures. Three Mabs against C (5A12, 5B11 and 5C6) neutralized the virus in primary CK cell culture but only two MAbs (5A12 and 5C6) against C protein neutralized the virus infectivity in Vero cell culture. Two MAbs (1A2 and 4D3) against C and 3 MAbs (4D1, 4E2, 5F5) against B/BC showed no neutralizing activities both in CK and Vero cell cultures. The neutralization of avian reovirus 1901 by MAbs against B/BC and C proteins indicated that the protein play important roles in infection processes and my be potential candidates for the preparation of a good vaccine.

Keyword :

neutralization, avian reovirus 1091, monoclonal antibodies, B/BC protein, C protein ,

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